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Okolab USA Inc microscope incubation chamber
Microscope Incubation Chamber, supplied by Okolab USA Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microscope+incubation+chamber/microscope+incubation+chamber/pm40281131-166-6-9
Average 90 stars, based on 1 article reviews
microscope incubation chamber - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gut-attached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO 2 , over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gutattached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO2, over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: Upregulation of the Nr2f1 - A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Article Snippet: Briefly, E12.5 and E13.5 whole intestines from control (G4-RFP) and mutant ( Spot Tg/Tg ;G4-RFP) embryos were placed on a small nitrocellulose filter (Millipore GSWP01300), which was then flipped on top of paraffin lines streaked in parallel on a 60-mm cell culture dish (Corning). .. Samples were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 10% FBS and penicillin-streptomycin for 6 h (at 37°C and 5% CO 2 ) in a microscope incubation chamber (Okolab), and 250-μm-thick stacks were acquired every 10 min using a Nikon A1R confocal unit and a 10× objective. ..

Microscopy:

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gut-attached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO 2 , over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: Coenzyme Q10 protects keratinocytes against oxidation-induced energy stress as revealed by spatiotemporal analysis of cell energetics.
Article Snippet: Fluorobrite medium contains 10% FBS, 1 mM L-glutamine, 50 μg/mL gentamicin (Gibco), and 100 U/mL penicillin and streptomycin (Gibco). .. The slides were placed into the microscope incubation chamber (OKOLAB) at 37 °C, 5% CO2 and 95% humidity for 1 h to reach perfectly stable conditions. ..

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gutattached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO2, over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: Upregulation of the Nr2f1 - A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Article Snippet: Briefly, E12.5 and E13.5 whole intestines from control (G4-RFP) and mutant ( Spot Tg/Tg ;G4-RFP) embryos were placed on a small nitrocellulose filter (Millipore GSWP01300), which was then flipped on top of paraffin lines streaked in parallel on a 60-mm cell culture dish (Corning). .. Samples were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 10% FBS and penicillin-streptomycin for 6 h (at 37°C and 5% CO 2 ) in a microscope incubation chamber (Okolab), and 250-μm-thick stacks were acquired every 10 min using a Nikon A1R confocal unit and a 10× objective. ..

Article Title: Ca V β controls the endocytic turnover of Ca V 1.2 L-type calcium channel.
Article Snippet: HEK293 cells transiently transfected with CaV1.2-HA and CaVβ were washed with phosphate buffered saline (PBS) and stained with mouse anti-HA tag DyLight 488 or DyLight 561 (1:100 dilution; Thermo Fisher) in Opti-MEM medium for 10 minutes at room temperature. .. Immediately after a second wash with PBS, cells were placed into the microscope incubation chamber (Okolab) at 37 C for live-cell imaging using spinning disc confocal microscopy. ..

Article Title: How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes
Article Snippet: .. During cell manipulation, cells were incubated at 37 °C and 5% CO 2 atmosphere in a microscope incubation chamber (Okolab, Pozzuoli, Italy). ..

Incubation:

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gut-attached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO 2 , over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: Coenzyme Q10 protects keratinocytes against oxidation-induced energy stress as revealed by spatiotemporal analysis of cell energetics.
Article Snippet: Fluorobrite medium contains 10% FBS, 1 mM L-glutamine, 50 μg/mL gentamicin (Gibco), and 100 U/mL penicillin and streptomycin (Gibco). .. The slides were placed into the microscope incubation chamber (OKOLAB) at 37 °C, 5% CO2 and 95% humidity for 1 h to reach perfectly stable conditions. ..

Article Title: A collagen VI–dependent pathogenic mechanism for Hirschsprung’s disease
Article Snippet: The gutattached filter paper was suspended, with the tissue side on paraffin bridges streaked in parallel on a 60-mm cell culture dish (Corning). .. The samples were cultured in DMEM/F12 containing 10% FBS and penicillin/streptomycin at 37°C, 5% CO2, over 10 hours in a microscope incubation chamber (Okolab). .. Image stacks (250-μm thick) were acquired every 10 minutes using a ×10 objective and a Nikon A1R confocal unit.

Article Title: Upregulation of the Nr2f1 - A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Article Snippet: Briefly, E12.5 and E13.5 whole intestines from control (G4-RFP) and mutant ( Spot Tg/Tg ;G4-RFP) embryos were placed on a small nitrocellulose filter (Millipore GSWP01300), which was then flipped on top of paraffin lines streaked in parallel on a 60-mm cell culture dish (Corning). .. Samples were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 10% FBS and penicillin-streptomycin for 6 h (at 37°C and 5% CO 2 ) in a microscope incubation chamber (Okolab), and 250-μm-thick stacks were acquired every 10 min using a Nikon A1R confocal unit and a 10× objective. ..

Article Title: Ca V β controls the endocytic turnover of Ca V 1.2 L-type calcium channel.
Article Snippet: HEK293 cells transiently transfected with CaV1.2-HA and CaVβ were washed with phosphate buffered saline (PBS) and stained with mouse anti-HA tag DyLight 488 or DyLight 561 (1:100 dilution; Thermo Fisher) in Opti-MEM medium for 10 minutes at room temperature. .. Immediately after a second wash with PBS, cells were placed into the microscope incubation chamber (Okolab) at 37 C for live-cell imaging using spinning disc confocal microscopy. ..

Article Title: How Localized Z-Disc Damage Affects Force Generation and Gene Expression in Cardiomyocytes
Article Snippet: .. During cell manipulation, cells were incubated at 37 °C and 5% CO 2 atmosphere in a microscope incubation chamber (Okolab, Pozzuoli, Italy). ..

Modification:

Article Title: Upregulation of the Nr2f1 - A830082K12Rik gene pair in murine neural crest cells results in a complex phenotype reminiscent of Waardenburg syndrome type 4
Article Snippet: Briefly, E12.5 and E13.5 whole intestines from control (G4-RFP) and mutant ( Spot Tg/Tg ;G4-RFP) embryos were placed on a small nitrocellulose filter (Millipore GSWP01300), which was then flipped on top of paraffin lines streaked in parallel on a 60-mm cell culture dish (Corning). .. Samples were cultured in Dulbecco's modified Eagle's medium (DMEM)/F12 medium containing 10% FBS and penicillin-streptomycin for 6 h (at 37°C and 5% CO 2 ) in a microscope incubation chamber (Okolab), and 250-μm-thick stacks were acquired every 10 min using a Nikon A1R confocal unit and a 10× objective. ..

Live Cell Imaging:

Article Title: Ca V β controls the endocytic turnover of Ca V 1.2 L-type calcium channel.
Article Snippet: HEK293 cells transiently transfected with CaV1.2-HA and CaVβ were washed with phosphate buffered saline (PBS) and stained with mouse anti-HA tag DyLight 488 or DyLight 561 (1:100 dilution; Thermo Fisher) in Opti-MEM medium for 10 minutes at room temperature. .. Immediately after a second wash with PBS, cells were placed into the microscope incubation chamber (Okolab) at 37 C for live-cell imaging using spinning disc confocal microscopy. ..

Confocal Microscopy:

Article Title: Ca V β controls the endocytic turnover of Ca V 1.2 L-type calcium channel.
Article Snippet: HEK293 cells transiently transfected with CaV1.2-HA and CaVβ were washed with phosphate buffered saline (PBS) and stained with mouse anti-HA tag DyLight 488 or DyLight 561 (1:100 dilution; Thermo Fisher) in Opti-MEM medium for 10 minutes at room temperature. .. Immediately after a second wash with PBS, cells were placed into the microscope incubation chamber (Okolab) at 37 C for live-cell imaging using spinning disc confocal microscopy. ..



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